Inhibitors | Comment | Organism | Structure |
---|---|---|---|
benznidazole | presents 80% inhibitory activity against amastigotes in LLC-MK2 cell culture infected with amastigote and trypomastigote forms of the parasite, leads to parasite recrudescence in trypomastigotes on the 15th day at 0.01 mM | Trypanosoma cruzi | |
hydroxymethylnitrofurazone | presents 100% inhibitory activity against amastigotes in LLC-MK2 cell culture infected with amastigote and trypomastigote forms of the parasite, leads to complete inhibition in trypomastigotes at 0.005 mM | Trypanosoma cruzi | |
additional information | AM1 semi-empirical molecular modeling studies analysing cruzain inhibitory activity, overview | Trypanosoma cruzi | |
nitrofurazone | a 5-nitro-2-furfurylidenesemicarbazone, presents 90% inhibitory activity against amastigotes in LLC-MK2 cell culture infected with amastigote and trypomastigote forms of the parasite, leads to parasite recrudescence in trypomastigotes on the 15th day at 0.005 mM | Trypanosoma cruzi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma cruzi | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Bz-Phe-Arg-4-methylcoumaryl-7-amide + H2O | - |
Trypanosoma cruzi | Bz-Phe-Arg + 7-amino-4-methylcoumarin | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Trypanosoma cruzi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
assay at | Trypanosoma cruzi |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0106 | - |
pH 6.5, 37°C | Trypanosoma cruzi | hydroxymethylnitrofurazone | |
0.0228 | - |
pH 6.5, 37°C | Trypanosoma cruzi | nitrofurazone |